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Inducible vancomycin resistance phenotype assay. Strain YSJ17 was tested for inducible vancomycin resistance by preincubation of 1/10 × MIC (0.2 mg/L) vancomycin for 1 h in Todd–Hewitt broth plus 0.2% yeast extract (THY) prior to dilution back to OD600 = 0.05 in the same medium containing 1/2 × MIC (1 mg/L) vancomycin. Growth lag was observed by the non-induced + 1/2 × MIC treatment and partial restoration was observed by the preincubated + 1/2 × MIC treatment.
