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. 2019 Sep 16;9:13397. doi: 10.1038/s41598-019-49635-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Platelet activators rapidly trigger monocyte TF exposure in whole blood. (a,b) PPACK-anticoagulated human whole blood was stimulated with PAR1-AP (1–100 µM; 5–25 mins) prior to staining with conjugated primary antibodies. Median fluorescence intensity (MFI) is shown for TF on CD14 + monocytes (a) and P-selectin on CD41a + platelets (b) (mean + S.E.M.; n = 5; n.s. not significant p > 0.05; *p < 0.05; **p < 0.01 compared to unstimulated at the same timepoint; Friedman’s non-parametric test with Dunn’s multiple comparison post-test). (c) Whole blood was stimulated with a range of concentrations of the platelet activators, CRP-XL, PAR4-AP, and ADP, for 10 minutes. Monocyte TF and platelet P-selectin are shown on the same axes for comparison of concentration-dependency of their effects.