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. 2019 Sep 16;9:13329. doi: 10.1038/s41598-019-49750-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic analysis of (A) P. dicentrarchi Cu/Zn-SODs and (B) Fe/Mn-SOD proteins by the Maximum Likelihood (ML) and Bayesian inference (BI) methods, respectively. The trees with the highest log likelihood (A) (−4402.19) and (B) (−1780.26) are shown. Evolutionary analysis was conducted with the MEGA7 and MrBayes 3.2.6. programs. Initial trees for the heuristic search were obtained automatically by applying Neighbor-Joining and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The analysis involved (A) 27 and (B) 19 AA sequences. Numbers on branches represent the bootstrap value for ML analysis (black) and posterior probability value of BI analysis (red). The codes that appear next to the species in parentheses are the GenBank sequence accession numbers. Dashes (−) indicate disagreement between the ML and BI analysis. The scale bar corresponds to 10 (A) or 20 (B) substitutions per 100 AA positions.