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. 2019 Sep 16;9:13329. doi: 10.1038/s41598-019-49750-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ELISA assay for the determination of expression levels of SODs in P. dicentrarchi trophonts exposed in vitro to ultraviolet (UV) radiation (3 J) relative to non-radiated trophonts (none). (A,B) The assay included polyclonal antibodies against the recombinant proteins of forms 2 and 3 of Cu/ZnSOD (anti-rCSD2 and anti-rCSD3, respectively), (C) Mn-SOD (anti-rMSD) and (D) Fe-SOD (anti-rFSD). The level of recognition was evaluated against the SODs present in peak 2 (P2) purified by ion exchange chromatography (see Fig. 1), using the proteins recombinants (rCSD2, rCSD3, rMSD and rFSD) as response controls and a sample of L5 culture medium to determine the presence of extracellular forms of SOD. The asterisks indicate statistically significant differences (P < 0.01), relative to the non-irradiated controls (determined by Student t test).