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. 2019 Sep 16;9:13329. doi: 10.1038/s41598-019-49750-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Expression of Cu/Zn-SOD3 (CSD3) in P. dicentrarchi trophonts exposed to different levels of UV radiation, as determined in a Western blot (WB) assay with a polyclonal antibody against the recombinant protein rCSD3 (anti-rCSD3) and proteins in peak 2 (P2) purified by ion exchange chromatography. The graph corresponds to the densitometric analysis of the WB (n = 3). A representative WB (not cropped) is shown in the figure. (B) Expression of Cu/Zn-SOD3 transcripts by ciliates exposed to different doses of UV radiation and quantified by RT-qPCR. (C) SOD activity in the P2 fraction from ciliates treated by UV irradiation (3 J/cm²) and untreated ciliates (control), as determined by the PMS-NBT (phenazine methosulfonate - nitroblue tetrazolium) assay. The enzymatic activity was quantified as the decrease in absorbance at 560 nm/min (−Δabsorbance/min). The mean values and standard error are represented in the bar graph, and the asterisks indicate statistically significant differences (*P < 0.05; **P < 0.01) relative to the non-irradiated control.