Figure 4.

Effect of the deletions of conserved and novel functional elements on the ability of TER to complement Δter mutation in Y. lipolytica. (a) A simplified scheme of Y. lipolytica TER with highlighted conserved domains and sequences subjected to functional analysis (for a more detailed structure of TER see Supplementary Fig. S5). (b–d) Wild-type (WT) and Δter strains were transformed with the plasmid constructs bearing the TER locus or its deletion variants (deletions of specific sequences are indicated), followed by the TRF analysis to test the ability of the plasmids to restore telomeric fragments in Δter mutant.