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. 2019 Sep 10;10:2039. doi: 10.3389/fmicb.2019.02039

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Copyright © 2019 Wang, Cai, Xing, Liu, Hendy and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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URM1 is responsible for modification of Ahp1. (A) Total urmylation level is responsive to oxidative stress. Total lysates of the WT/URM1FLAG strain under different conditions were subjected to SDS-PAGE and immunoblotted using anti-flag antibodies to detect total urmylation levels. (B) Ahp1 is modified by URM1. Ahp1:3 × Flag fusion constructs were transformed into the wild type and the Δurm1 mutant, respectively. Corresponding transformants cultured with or without 1.5 mM diamide were analyzed by western blotting with anti-flag antibody. Triangle indicates the Ahp1:3 × Flag fusion protein bands. The β-actin levels detected by anti-actin were used as a loading control.