Figure 2.

Germination and alternative splicing regulation by red/far-red light. (A-D) Germination induced by an Rp reduces the splicing index (SI) of At-U2AF65A, At-SR30, At-RS31, and At-RS31a in Col-0 seeds. We selected these genes from the RNA-seq data based on two simultaneous criteria: (1) genes involved in RNA splicing and (2) genes previously documented to have AS (Petrillo et al., 2014; Shikata et al., 2014). RNA Col-0 samples were harvested 12 h after the Rp or FRp. AS alterations as SI in response to the Rp or FRp are shown. Gene expression levels were analyzed by RT–PCR, and each PCR product was quantified by densitometry using ImageJ. Each bar represents mean ± SE (n = 3). Significant differences between means are shown by different letters (p < 0.05 by Student’s t-test). AS, alternative splicing; Col-0, Columbia-0; FRp, far-red pulse; Rp, red pulse; RT–PCR, reverse transcriptase–polymerase chain reaction.