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. 2019 Sep 16;9:13416. doi: 10.1038/s41598-019-49933-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo analysis following islet transplantation: (a) In vivo experimental details; (b) An example of an islet transplant (both control and islets treated with pFUS) using the kidney sub-capsule space as the site of transplantation: islets before, during and after transplantation (black circle = transplanted islets); pFUS treatment on the kidney containing the transplanted islets (red circle = site of pFUS targeting). Results of (c) blood glucose measurements, (d) normoglycemia percentage, (e) body weight, (f) IPGTT, (g) area under the IPGTT curve (AUC_0-120min), and (h) blood glucose clearance rates calculated from the slope of IPGTT curves from 30 to 90 min. Significant differences: (c–h) ^aP < 0.05: islets only vs. islets treated with pFUS; *P < 0.05: baseline vs. all other time-points (Two (c,e,f) or one (b)-way ANOVA with post-hoc Tukey Test or unpaired Student’s t-test (g,h)).