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. 2019 Sep 16;9:13416. doi: 10.1038/s41598-019-49933-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological assessment of transplanted islets: (a) Representative images following H&E, insulin, vWF and TNF-α immunohistochemical staining of islets that were transplanted under the kidney capsule. Black arrows = islets; Red arrow = positive (dark brown) staining; (b) Quantification of the surface area occupied by islets; (c–e) Quantification of positive (c) insulin, (d) vWF and (e) TNF-α staining. For all figures, control samples (non-treated islets) are compared to islets treated with pFUS. Significant differences: *P < 0.05 for islets only vs. islets treated with pFUS (Student’s unpaired t-test).