FIGURE 4.

Single vagal afferent firing was reduced in aged mice but could be rescued. (A) Mean interval between spikes of vagal afferents were longer in aged mice, indicating a reduction in vagal afferent firing rate in the aged mice (N = 148 fibers). (B) Intraluminal squalamine (10 μM) reduced the mean interval between vagal spikes in young mice (N = 38 fibers). (C) Intraluminal squalamine (10 μM) reduced the mean interval between vagal spikes in aged mice, increasing firing frequency toward that of the young controls (N = 22 fibers). Data represented as mean with SEM, two-tailed t-test unpaired, with Welch’s correction and paired, two-tailed t-tests.