FIGURE 5.

Response onset to squalamine is longer in old mice. (A) Spike frequency histograms show changes in spike frequency over the course of the experiment. Onset to peak squalamine response in vagal afferent fibers in a young mouse after addition of squalamine at 12 min. (B) Response onset to squalamine in vagal afferent fibers in an old mouse after addition of squalamine. (C) Response onset to squalamine on vagal afferent firing rate was longer in old mice [N = 4(22)] compared to young mice [N = 3(38)]. Data represented as mean with SEM, t-test unpaired, with Welch’s correction, two-tailed.