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. 2019 Sep 10;10:2105. doi: 10.3389/fmicb.2019.02105

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Copyright © 2019 Lou, Zhao, Tang, Ye, Wei, Lin and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study on the function of the Cmtns gene. (A) The conidia of the ΔCmtns mutant were co-cultured with the Agrobacterium tumefaciens strain AGL1-pCAMBIA0390-Ben-Comtns on PDA medium supplemented with 3 μg/mL of benomyl, and the co-culture plate for complementation of the Cmtns gene was obtained. (B) PCR amplification of the ben gene in the ΔCmtns-c strain. (C) PCR amplification of the Cmtns gene in the ΔCmtns-c strain. (D) qRT-PCR analysis of the Cmtns gene expression. (E) C. militaris CM10 cultured on rice medium. (F) C. militaris ΔCmtns cultured on rice medium. (G) C. militarisΔCmtns-c cultured on rice medium. (H) Analysis of carotenoid content in C. militaris. Data are means ± standard error from three independent experiments. Different letters (a–c) above the bars indicated significant difference (ANOVA followed by Duncan’s multiple-range tests, P < 0.05).