Figure 3.

Enhanced control of mycobacterial infection in the lung by cognate Ag-based RM-pull strategy in parenteral vaccine-primed animals. (A) Experimental schema. Mice were left untreated, only immunized i.m. with AdCh68Ag85A (i.m.), or immunized i.m. and received i.n. delivery of CpG (single dose) (i.m. CpG) or Ag85 complex proteins (two doses 5 days apart) (i.m. Ag85) at day 33 or day 28 post-immunization. Some mice were sacrificed before infection for T cell analysis and other groups of mice were infected with M.tb H37Rv and the lungs were harvested for analysis 2 weeks after infection. (B) Representative dotplots and bar graph comparing frequencies of circulating Ag85A-specific CD8 T cells in i.m., i.m./CpG, and i.m./Ag85 groups (C) Bar graph showing changes in the level of M.tb infection in the lung of i.m. CpG and i.m. Ag85 groups. Data are expressed as the mean ± S.E.M of fold changes relative to i.m. group from 10 mice/group of two independent experiments. *P < 0.05 compared with CpG group.