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. 2019 Sep 10;10:2119. doi: 10.3389/fmicb.2019.02119

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Copyright © 2019 Liu, Bogaj, Bortolaia, Olsen and Thomsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of SOS-responsive genes in the transconjugants from the first round of conjugation. Cell samples grown with no or 1/2 MIC of antibiotics (+CTX, AMP, or CIP) were used for RT-qPCR. Data are presented as fold change relative to control without antibiotics. Note that the different figures are not drawn to the same scale. The combination of strain number with antibiotic name represents the transconjugants from the first round obtained after exposure to the specified antibiotic. The expression data were normalized to two validated reference genes, gapA and nusG. The results shown are means of three biological replicates with two technical replicates each and the error bars represent standard errors of the means. The stars indicate statistical significance at different levels: ^∗P ≤ 0.05, ^∗∗P ≤ 0.01.