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. 2019 Aug 26;20(17):4184. doi: 10.3390/ijms20174184

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The partitioning of the 12.5 µM of tachyplesin I–III (TI-TIII) and cyclic analogues (cTI-cTIII) into lipid membranes. (a) Normalized fluorescence emission spectra of cTI in aqueous solution (grey) and upon titration with LUVs composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; up to 3 mM) or of POPC/POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine; 4:1) up to 1 mM). Fluorescence emission spectra were obtained with excitation at 280 nm (b) Wavelength at which TI (solid symbol) and cTI (open symbol) have their maximum fluorescence emission in buffer and with increasing concentrations of LUVs composed of different lipid mixtures (POPC: red; POPC/POPS (4:1): blue; POPC/POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol; 4:1): green).