Table 1.
The sequence and physicochemical properties of tachyplesin I–III (TI-TIII) and their cyclic analogues (cTI-cTIII).
| Peptide | Sequence 1 | Mass (Da) 2 | RT (min) 3 | Charge 4 | |
|---|---|---|---|---|---|
| Calc. | Obs. | ||||
| TI | KWCFRVCYRGICYRRCR * | 2263.8 | 2263.5 | 18.04 | +7 |
| TII | RWCFRVCYRGICYRKCR * | 2263.8 | 2263.5 | 17.79 | +7 |
| TIII | KWCFRVCYRGICYRKCR * | 2235.8 | 2235.6 | 17.68 | +7 |
| cTI | KWCFRVCYRGICYRRCRG | 2303.8 | 2303.7 | 18.69 | +6 |
| cTII | RWCFRVCYRGICYRKCRG | 2303.8 | 2303.7 | 18.53 | +6 |
| cTIII | KWCFRVCYRGICYRKCRG | 2275.8 | 2275.5 | 18.44 | +6 |
1 Peptide sequences and amino acid residues differing from TI are in bold. * denotes C-terminal amidation. 2 Average mass calculated (Calc.) from the amino acid sequence and experimentally observed (Obs.) using synthetic peptide and determined from m/z 3+ in ESI-MS. 3 Retention time (RT) of peptides on an analytical RP-HPLC; chromatograms shown in Supplementary Figure S1a were obtained with a 2%/min gradient of 0–40% solvent B (90% acetonitrile; 0.05% trifluoroacetic acid (TFA) (v/v)) in solvent A (H2O, 0.05% TFA (v/v)) at a flow rate of 0.3 mL/min. 4 Charge of the peptides at pH 7.4.