Table 7.
Shift in the fluorescence emission maximum wavelength of tachyplesin I–III (TI-TIII) and cyclic analogues (cTI-cTIII) in buffer and in the presence of model membranes.
| POPC | POPC/POPS (4:1) | POPC/POPG (4:1) | |||
|---|---|---|---|---|---|
| Peptide | shift (nm) 1 | shift (nm) | 0.5 [L] (mM) 2 | shift (nm) | 0.5 [L] (mM) |
| TI | 2 | 22 | 0.19 | 30 | 0.09 |
| TII | 0 | 19 | 0.30 | 29 | 0.09 |
| TIII | −3 | 18 | 0.30 | 23 | 0.10 |
| cTI | 0 | 28 | 0.11 | 28 | 0.06 |
| cTII | 1 | 27 | 0.08 | 29 | 0.05 |
| cTIII | 3 | 27 | 0.12 | 28 | 0.08 |
1 blue shifts observed with 3 mM POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine); 1 mM POPC/POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine; 4:1), or 1 mM POPC/POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol; 4:1) LUVs. 2 lipid concentrations required to achieve half of the maximum blue shift observed (0.5 [L] in mM).