Table 9.
Fluorescence quenching of tachyplesin I (TI) and and cyclic tachyplesin I (cTI) partitioned into 1 mM POPC/POPS (4:1) LUVs by the quenchers 5DS and 16DS. 1.
| 5DS | 16DS | ||||
|---|---|---|---|---|---|
| Peptide | KSV (M−1) | fb | KSV (M−1) | fb | Z (Å) 2 |
| TI | 3.7 ± 1.3 | 0.63 ± 0.57 | 19.6 ± 4.3 | 0.74 ± 0.37 | 8.8 |
| cTI | 4.9 ± 1.8 | 0.61 ± 0.54 | 26.3 ± 7.1 | 0.83 ± 0.52 | 5.5 |
1 The KSV and fb (the fraction of light emitted by the peptide accessible to the quencher) were determined using the Lehrer equation (Equation (2)). 2 The calculated average distance, Z in Å, of the Trp residue from the bilayer center was determined using the parallax method (Equations (3) and (4)) [62].