Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 24;20(17):4139. doi: 10.3390/ijms20174139

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Purification and characterization of the thermostable keratanase. (A) SDS-PAGE of thermostable keratanase. The purified thermostable keratanase was applied to a 10% Tris-Glycine SDS-PAGE gel, and the proteins were visualized by Coomassie Brilliant Blue G-250 staining. Lane 1, markers; lane 2, purified enzyme (5 mU, 0.4 μg). (B) Treatment with 1 μU/mL purified thermostable keratanase degraded KS chains but not diHS-0S, diHS-NS and C6S chains in MPS IVA chondrocyte cells from 3-dimensional (3D) culture; n = 3. Statistics were analyzed by unpaired t-test. Data are presented as mean ± SD. * p < 0.05. C6S: chondroitin 6 sulfate, HS: heparan sulfate, KS: keratan sulfate, NT: untreated, TSK: thermostable keratanase.