Figure 7.

The possible regulation manner of the cellulase gene transcription by Are1 in T. reesei. (A) Validation of the transcription levels of the cellulase genes, cbh1 and cbh2, in the T. reesei strains, which were grown in the MM medium using 10 mM (NH₄)₂SO₄ as the sole nitrogen source. (B) The transcription levels of the major cellulase transcription factors, Xyr1 and Cre1, in the T. reesei strains. RT-qPCR analysis was performed to detect the transcript abundance in (A) and (B). Error bars indicate the standard deviation and ** shows a p-value < 0.01. (C) Putative DNA binding sites of Are1 in promoter regions (within 1000 bp) of cbh1 and cbh2. White triangle, HGATAR (where H stands for A, T, or C and R stands for A or G); Black triangle, YTATCD (where Y stands for T, G, or A, and D stands for T or C).