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. 2019 Aug 27;20(17):4203. doi: 10.3390/ijms20174203

Figure 3.

Figure 3

Normoxia stimulates the expression of genes associated with glucose metabolism and to the lower extent with glutamine metabolism in cell line-dependent manner. (A) Transcript levels of GLUT1 (glucose transporter 1), PDK1 (pyruvate dehydrogenase kinase 1) and HK2 (hexokinase 2) in melanoma cells incubated in the presence of 21% O2, 6% O2 or 1% O2 for 24 h were determined by qRT-PCR and normalized to the expression of a reference gene RPS17. Gene expression in 6% O2 and 1% O2 is presented relative to the expression in 21% O2. (B) Transcript levels of GLUT1, PDK1 and HK2 in melanoma cells cultured in the presence of 6% O2 for at least 3 weeks (established 6% O2 culture) relative to their levels in cells cultured in 21% O2. (C) Transcript levels of GLS (glutaminase), SLC1A5 (solute carrier family 1 member 5) and SLC7A11 (solute carrier family 7 member 11 transporter) in melanoma cells after 24 h incubation in 21% O2, 6% O2 and 1% O2, or (D) in the established 6% O2 culture, relative to their levels in 21% O2. Bars represent mean values of 3-4 biological replicates ± SD. Differences are considered significant at * p < 0.05, ** p < 0.01 or *** p < 0.001.