Figure 4.

KAR-mediated depression of glutamate release does not require changes in Ca²⁺ in the cytosol at the PF-PuC synapses. (A) Time-course of KA (3 μM) effect on eEPSC amplitude under the control condition (circles) and in slices treated with Ryanodine (squares). (B) Quantification of results for slices treated with ryanodine, thapsigargin and Philanthotoxin. The decrease of eEPSC amplitudes induced by KA is not prevented in any case. The number of slices (from two to three mice) is indicated in parentheses at the top of each bar. Results are expressed as means ± SEM (Student’s t-test and ANOVA).