Figure 11.

Diatrizoic acid effects on protein carbonylation in HK-2 cells. Carbonylated proteins were similar between control and DA treated cells at 8 h (A). DA at 18–30 mg I/mL increased protein carbonylation in cell lysate at 24 h (C). Memcode reversible stain for 15 µg protein depicted for 8 h (B) and 24 h (D) exposure. Statistical difference from 0 mg I/mL diatrizoic acid loading for gels is depicted by an asterisk (* p < 0.05, ** p < 0.01, *** p < 0.001). Values represent mean ± SEM for three independent experiments.