Figure 12.

Diatrizoic acid effects on 4-hydroxynonenol (4HNE) adduct formation in HK-2 cells. An increase in 4HNE protein adduct formation was evident in cell lysate following 24 h exposure (B) to 18–30 mg I/mL. Positive bands of 4HNE were unchanged after 8 h (A) DA exposure. Panels (C) and (D) depict 8 h and 24 h, respectively, for protein loading of 40 µg per lane. Statistical difference from 0 mg I/mL diatrizoic acid depicted by an asterisk (* p < 0.05). Values represent mean ± SEM for three independent experiments.