Figure 13.

Diatrizoic acid effects on superoxide dismutase expression and activity in cellular fractions of HK-2 cells. Total superoxide dismutase (SOD) activity was decreased at 24 h by 28 and 30 mg I/mL (A). DA exposure did not change MnSOD activity (B) or protein expression (D). A decrease in Cu/Zn activity (C) was evident at 24 h exposure to 23–30 mg I/mL. Protein staining with Memcode (E) shown for blot visualizing MnSOD (D). Statistical difference from 0 mg I/mL diatrizoic acid depicted by an asterisk (* p < 0.05, ** p < 0.01). Values represent mean ± SEM for three independent experiments.