Figure 14.

Diatrizoic acid effects on oxidative stress within cellular fractions of HK-2 cells. DA-induced oxidative stress predominantly in the cytosol. Representative blot and cumulative densitometry for expression of Oxyblot (A) and 4HNE protein adducts (C) in cytosolic and mitochondrial fractions following 24 h exposure to DA. Memcode reversible staining of blots loaded with 15 and 40 µg protein and densitometry for Oxyblot (B) and 4HNE (D). Asterisks (* p < 0.05) indicate statistical difference from vehicle control in cytosolic fraction. Values represent mean ± SEM for three independent experiments.