Figure 16.

Diatrizoic acid effects on mitochondrial membrane integrity in HK-2 cells. DA diminished mitochondrial membrane integrity. Representative blot and cumulative densitometry for cytochrome c expression (A) in cytosolic and mitochondrial fractions following 24 h exposure to DA. Representative blot of protein staining to confirm equal 40 µg protein loading for cytochrome c (B). Asterisks (* p < 0.05, ** p < 0.01) indicate statistical difference from vehicle control in cytosolic fraction. Octothorpe (# p < 0.05) indicate statistical difference from vehicle control in mitochondrial fraction. Values represent mean ± SEM for three independent experiments.