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. 2019 Aug 21;20(17):4074. doi: 10.3390/ijms20174074

Figure 17.

Figure 17

Diatrizoic acid effects on the expression of caspase 4, caspase 12, and caspase 3 in HK-2 cells. DA induced apoptosis via caspase 3 and caspase 12 activation. Representative blot and cumulative densitometry for total caspase 4 (A), caspase 12 (B), and cleaved caspase 3 (C) protein expression following 24 h exposure to DA. Asterisks (* p < 0.05, ** p < 0.01) indicate statistical difference from vehicle control. Protein loading reversible stain shown below respective protein. Each lane was loaded with 40 µg protein. Values represent mean ± SEM for three independent experiments.