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. 2019 Aug 21;20(17):4074. doi: 10.3390/ijms20174074

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of BAPTA-AM or calpeptin pretreatment on light chain (LC)3B expression in HK-2 cells. Pretreating HK-2 cells with BAPTA-AM or calpeptin attenuated DA-induced conversion of LC3BI to LC3BII. Representative blots and cumulative densitometry included for LC3BI (A), LC3BII (B), and LC3BII/LC3B I ratio (C) following 24 h exposure to DA. Protein loading stain depicted for 40 µg loaded pin (D). Positive control for LC3B conversion was FCCP (oxidative phosphorylation uncoupling agent). Different superscripts (a–c) indicate a statistical difference (p < 0.05) between group. Values represent mean ± SEM for three independent experiments of two biological replicants.