Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 29;20(17):4233. doi: 10.3390/ijms20174233

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Examples of expression of cell surface target antigens on CD34⁺ stem cells in normal bone marrow (NBM) and patients with acute myeloid leukemia (AML). Target expression on aspirated CD34⁺/CD38⁻ cells (left panels, red histograms) and CD34⁺/CD38⁺ BM cells (right panels, blue histograms) was determined by fluorochrome-conjugated antibodies (as depicted) and multi-color flow cytometry. Normal/reactive BM (NBM) was obtained from lymphoma patients without BM involvement or was purchased, and leukemic BM was obtained from three patients with AML. All patients gave written informed consent before BM aspiration was performed. The study was approved by the ethics committee of the Medical University of Vienna. Reactivity of the test antibodies (CD34⁺/CD38⁻ stem cells: red histograms; CD34⁺/CD38⁺ stem/progenitor cells: blue histograms) was assessed on a FACSCantoII (BD Biosciences). Antibody reactivity was controlled by isotype-matched control antibodies (open black histograms). Flow cytometry data were analyzed using FlowJo 8.8.7 software (Flowjo).