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Zm-INVINH4 is targeted in the apoplast. (a)Transient expression of Zm-INVINH4::GFP and Zm-INVINH1::RFP under the control of the cauliflower mosaic virus 35S promoter in onion epidermal cells. (b) Immunoblot of Zm-INVINH4::HA by using aiti HA-tag antiserum in transient expressed Nicotiana benthamiana leaves. SP, soluble fraction; TP, crude pellet immediately boiled; SE, salt elution; TSE, insoluble pellet after salt elution.
