Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 29;20(17):4226. doi: 10.3390/ijms20174226

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(A) The effects of KP on EGF receptor (EGFR) expression in HeLa cells treated with different concentrations of KP or DMSO for 24 h; (B) Western blotting with quantitative analysis of total EGFR in HeLa cells treated with different concentrations of KP or DMSO for 24 h; (C) immunofluorescence for EGFR phosphorylation (pEGFR) at tyrosine 1068 (Y1068) (green) in HeLa cells treated with different concentrations of KP for 24 h and stimulated with EGF for 2 min; (D) immunofluorescence for ERK1/2 phosphorylation (green) in HeLa cells treated with different concentrations of KP for 24 h and stimulated with EGF for 15 min. Nuclei were counterstained with Hoechst 33342 (blue); (E) Western blot analysis for EGFR phosphorylation at Y1045 and Y1068 and pERK1/2 in HeLa cells treated with different concentrations of KP for 24 h and stimulated with EGF for 2 min; (F) quantitative analysis for EGFR phosphorylation at Y1068; (G) quantitative analysis for EGFR phosphorylation at 1045; (H) quantitative analysis for ERK1/2 phosphorylation. Micrographs were captured at 40× magnification. Data represent mean ± SD of three replicates. * p < 0.05.