Figure 6.

The effects of KP on suppressing IL-6-induced STAT3 signaling in HeLa cells. (A) Immunofluorescence for nuclear STAT3 phosphorylation (red) in HeLa cells treated with KP at 15 μg/mL, with the presence of human recombinant IL-6 (50 ng/mL) for 24 h. The total STAT3 protein (green) was detected to visualize total expression of STAT3; (B) Western blotting and quantitative analysis for pSTAT3, Mcl-1, and pAkt in HeLa cells treated with different concentrations of KP or DMSO, with the presence of human recombinant IL-6 for 24 h. Micrographs were captured at 40× magnification. Data represent mean ± SD of three replicates. * p < 0.05.