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. 2019 Aug 27;20(17):4202. doi: 10.3390/ijms20174202

Figure 2.

Figure 2

NaHS suppressed the osteogenic transition, Stat3 phosphorylation, activities and protein expression of cathepsin S (CAS), and increased the protein expression of elastin (G) of HG-cultured HASMCs. Western blots showing that NaHS attenuated HG-induced down-regulation in SMα-actin (A), SM22α (B), and elastin (G) and up-regulation of Cbfα-1 (C), Stat3 phosphorylation (D) and activities and protein expression of CAS (E,F). n = 3–5. Values represent the means ± SEM. * p < 0.05 compared to LG. # p < 0.05 compared to HG. The protein expressions of SMα-actin, SM22α, elastin, Cbfα-1 Stat3, CAS, and elastin were examined in HG-cultured HASMCs after NaHS (100 μM) treatment for 7 days. β-actin or total Stat3 from the same blots was used as a protein loading control.