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Free energy landscape (kJ mol−1) as a function of the first two dihedral principle components (dPC1 and dPC2); the lowest energy conformations of each family as identified by k-means clustering are shown. Secondary structural motifs and ions are shown (blue, α-helix; red, β-sheet; green, β-turn/bend; aqua, coil; pink, Zn2+; and yellow, Ca2+).
