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. 2019 Sep 2;20(17):4293. doi: 10.3390/ijms20174293

Figure 3.

Figure 3

hnRNPA1 mediates the anti-tumor effects of Quercetin. (A) Thyroid and pancreatic cancer cells were treated with increasing concentrations of Quercetin for 24 h, and the cells were analyzed for hnRNPA1 and GAPDH (loading control) by western blot analysis. (B) Cancer cells transfected with control vector or vector expressing hnRNPA1 were treated with DMSO or a combination of JQ1 (JQ, 1 µmol/L) and Quercetin (Quer, 20 µmol/L) for 24 h. Expression of flag-tagged hnRNPA1 and the effect on c-PARP-1 was determined by western blot analysis using HSP90 as loading control. The results are representative of three independent experiments. hnRNPA1: heterogeneous nuclear ribonucleoprotein A1; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase (loading control); c-PARP1: cleaved PARP1, HSP90: heat shock protein 90 (loading control); DMSO: dimethyl sulfoxide; JQ1: BET inhibitor; K1, MDA-T85: human papillary thyroid cancer cell lines; 8505c: human anaplastic thyroid cancer cell line; CD18: human pancreatic cancer cell line.