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. 2019 Sep 2;20(17):4293. doi: 10.3390/ijms20174293

Figure 4.

Figure 4

Co-treatment of Quercetin and JQ1 decreases Survivin. (A) Pancreatic cancer cells were treated with DMSO, JQ1 (1 µmol/L), Quercetin (Quer, 20 µmol/L), or a combination of JQ1 (1 µmol/L) and Quercetin (20 µmol/L) for 24 h. Cell lysates were collected and analyzed for apoptosis-related proteins using the Proteome Profiler Human Apoptosis Array ARY009, and the pixel density of Survivin from the array data was quantified by ImageJ. Error bars represent SD from two technical replicates. The results are representative of two independent experiments. Survivin is highlighted with white boxes. (B) Cells were treated with DMSO, JQ1 (1 µmol/L), Quercetin (20 µmol/L), or a combination of JQ1 (1 µmol/L) and Quercetin (20 µmol/L) for 24 h. The effect on c-PARP1 and Survivin was determined by western blot analysis, using HSP90 as loading control. The results are representative of three independent experiments. DMSO: dimethyl sulfoxide; JQ1: BET inhibitor; c-PARP1: cleaved PARP1; HSP90: heat shock protein 90 (loading control); SD: standard deviation; K1: human papillary thyroid cancer cell lines; 8505c: human anaplastic thyroid cancer cell line; CD18: human pancreatic cancer cell line.