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. 2019 Aug 24;20(17):4135. doi: 10.3390/ijms20174135

Figure 1.

Figure 1

Flowchart of the methodology used for the total and plasma membrane proteome analysis. (A) Pipeline for the TMT based proteomics approach for proteome analysis of cytosolic and plasma membrane samples after MSP1 treatment. (B,C) Methodology for the normalization of protein intensities within 10-plex kit and between separate LC-MS runs.(D) SDS-PAGE and Western blotting using organelle specific marker proteins, Glutamine synthase (GS) as cytosolic marker, Histone 1 (H1) as nuclear marker and Plasma membrane intrinsic protein 2 (PIP2) as plasma membrane marker.