Table 2.
Standard curve statistics.
| Compound | Injected Concentration (ng/mL) | Retention Time (min) | Measured Concentration (ng/mL) | Area Under Peak | Straight Line Equation | LOD and LOQ (ng/mL) * | Lowest X Value Used (ng/mL) ** | R2 | % RSD |
|---|---|---|---|---|---|---|---|---|---|
| Nicotine | 1400.00 ng/mL | 13 | 1409 | 45,034 | Y = 32X − 560 | 122 and 369 | 150,203 | 1 | 6.7 |
| 700.00 ng/mL | 13.1 | 658 | 20,573 | ||||||
| 140.00 ng/mL | 13.1 | 164 | 4695 | ||||||
| Nornicotine | 1400.00 ng/mL | 8 | 1403 | 29,386 | Y = 21X − 332 | 18 and 54 | 410 | 1 | 4.2 |
| 700.00 ng/mL | 8.1 | 694 | 14,368 | ||||||
| 140.00 ng/mL | 8.1 | 143 | 2706 | ||||||
| Cotinine | 1400.00 ng/mL | 8.8 | 1393 | 97,916 | Y = 70X + 478 | 46 and 139 | 227 | 1 | 2.4 |
| 700.00 ng/mL | 8.8 | 716 | 50,563 | ||||||
| 140.00 ng/mL | 8.8 | 131 | 9651 | ||||||
| Anabasine | 1400.00 ng/mL | 10 | 1385 | 48,629 | Y = 35X + 753 | 94 and 286 | 322 | 1 | 3.7 |
| 700.00 ng/mL | 10 | 733 | 26,100 | ||||||
| 140.00 ng/mL | 10.1 | 121 | 4951 | ||||||
| Anatabine | 1400.00 ng/mL | 10.6 | 1389 | 18,417 | Y = 13X + 193 | 69 and 208 | 416 | 1 | 2.7 |
| 700.00 ng/mL | 10.6 | 724 | 9685 | ||||||
| 140.00 ng/mL | 10.6 | 127 | 1856 | ||||||
| .Myosmine | 1400.00 ng/mL | 11.7 | 1392 | 109,515 | Y = 78X + 971 | 50 and 153 | 674 | 1 | 1.96 |
| 700.00 ng/mL | 11.7 | 718 | 56,923 | ||||||
| 140.00 ng/mL | 11.7 | 1308 | 11,123 |
* Limit of detection (LOD) and limit of quantitation (LOQ) were determined based on the calibration curve. ** Lowest X value used from all sample chromatograms. RSD = relative standard deviation (i.e., Coefficient of variation).