. 2019 Aug 27;20(17):4200. doi: 10.3390/ijms20174200

Table 2.

GEFIs for inorganic ions measuring.

Name	Analyte	Permutant	Sensor Unit	λex, nm	λem, nm	Kd	RA	Reference
ZnGreen2	Zn²⁺	cpmTFP	Zinc hook peptide from Pyrococcus furiosus Rad50	462	492	20 mM	8.7-fold	[139]
Am Tryoshka 1;3 LS-F138I	NH⁴⁺	cpsfGFP, LSSmOrange	AtAMT1;3	440/480	510/570	84.5 μM	−15–22% green channel	[73]
Am Tryoshka 1;3 LS-F138I-T78H	NH⁴⁺	cpsfGFP, LSSmOrange	AtAMT1;3	440/480	510/570	64.4 μM	−30% green channel	[73]
GCS-2	Cu²⁺	cpGFP	ATCUN motif (Gly-Gly-His)	480	510	8 nM	85%	[135]
GZnP1	Zn²⁺	cpGFP	2 zinc fingers of the yeast transcription factor Zap1 (ZF1 and ZF2)	496	512	NM	15%	[138]

The raw colors refer to the colors of fluorescent proteins used as reporter domains. Kd—dissociation constant. NM—not measured. RA—response amplitude.