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. 2019 Aug 27;20(17):4200. doi: 10.3390/ijms20174200

Table 5.

GEFIs for organic metabolites measuring.

Name Analyte Permutant Sensor Unit λex,nm λem,nm Kd RA Reference
MBP165-cpAzurite Maltose cpAzurite MBP NM 440/480 3.3 μM 1.5-fold [194]
MBP165-cpCFP Maltose cpCFP MBP NM 500 13 μM 1.75-fold [194]
iGlucoSnFR Glucose cpGFP TtGBP 488 520 7.7 mM 3.3-fold [185]
iGlucoSnFR-mRuby2 Glucose cpGFP TtGBP 488/561 520/600 2.2 mM 2.0-fold [185]
iGlucoSnFR-TS (Sweetie TS) Glucose cpT-Sapphire TtGBP 400/480 510 FLIM-probe [186]
CF98 Citrate cpGFP CitA 413/504 518 0.1–50 mM citrate detection 2-fold [92]
N-MBP:cGFP:C-MBP Maltose cpGFP TmMBP ≈490 NM 6.89 mM 1.2-fold [193]
MBP165-cpGFP Maltose cpGFP MBP NM ≈510 4.5 μM 2.5-fold [194]
EcPhnD90-cpGFP. L1ADΔΔ.L297R,L301R 2-AEP cpGFP PhnD NM NM 37±7 μM 2.6-fold [96]
QUEEN-7μ ATP cpGFP ε-F0F1 400/494 513 7 μM NM [187]
QUEEN-2m ATP cpGFP ε-F0F1 400/494 513 4.5 mM NM [187]
iATPSnFR1.0 ATP cpSFGFP ε-F0F1 490 512 120 μM 3.4-fold [188]
iATPSnFR1.1 ATP cpSFGFP ε-F0F1 490 512 50 μM 2.9-fold [188]
mRuby- iATPSnFR1.0 ATP cpSFGFP ε-F0F1 490/NM 512/NM The same as for iATPSnFR1.0 [188]
MBP165-cpYFP Maltose cpYFP MBP NM 525 3.3 μM 3-fold [194]
FGBP1mM Glucose cpYFP GGBP 420/500 515 1 mM 7-fold [93]
GEVALs GTP cpYFP FeoB ≈400/485 NM 30 μM, 260 μM, 530 μM, 1150 μM, 2300 μM 2-fold [101]
FHisJ Histidine cpYFP HisJ 420/500 515 22 μM 6.2-fold [95]
Perceval [ATP]:[ADP] cpVenus MjGlnK1 405/490 520 KR = 0.5 2-fold [80]
PercevalHR [ATP]:[ADP] cpVenus MjGlnK1 420/500 520 KR = 3.5 > 8-fold [104]

The raw colors refer to the colors of fluorescent proteins used as reporter domains. Kd—dissociation constant. NM—not measured. RA—response amplitude.