Table 6.

GEFIs for secondary messengers measuring.

Name	Analyte	Permutant	Sensor Unit	λex,nm	λem, nm	Kd	RA	Reference
Upward DAG	DAG	cpGFP	PKCδ without C2 domen	NM	NM	NM	45% (in vivo)	[202]
Downward DAG	DAG	cpGFP	PKCδ without C2 domen	NM	NM	NM	−40% (in vivo)	[202]
Upward DAG2	DAG	cpGFP	PKCδ without C2 domen	NM	NM	Improved version of Upward DAG		[26]
Downward DAG2	DAG	cpGFP	PKCδ without C2 domen	NM	NM	Improved version of Downward DAG		[26]
cADDis	cAMP	cpGFP	EPAC2	NM	NM	10–100 μM cAMP detection	−35% (in vivo)	[205]
cAMPr	cAMP	cpGFP	PKA-catalytic and PKA regulatory subunit Iα (RIα)	490	520	NM	1.5-fold	[79]
α-FlincG	cGMP	cpEGFP	Regulatory domain PKG Iα	480	510	35 nM	1.55-fold	[87]
β-FlincG	cGMP	cpEGFP	Regulatory domain PKG Iβ	480	510	1.1 μM	2.05-fold	[87]
δ-FlincG	cGMP	cpEGFP	Regulatory domain PKG Iα	410/480	510	490 nM	3.5-fold	[87]
FlincG2	cGMP	cpEGFP	Regulatory domain PKG Iα	480	510	Modified version of δ-FlincG		[208]
FlincG3	cGMP	cpEGFP	Regulatory domain PKG Iα	480	510	Modified version of δ-FlincG		[208]
Btk-cpGFP	inositol-1,3,4,5-tetrakisphosphate	cpGFP	PH domain from Btk	396/470	508	100 nM	1.5-fold	[67]
R-FlincA	cAMP	cp146mApple	high-affinity cAMP-binding motif from the human PKA regulatory subunit Iα (RIα)	571	590	0.3 μM	860%	[209]

The raw colors refer to the colors of fluorescent proteins used as reporter domains. Kd—dissociation constant. NM – not measured. RA—response amplitude.