Table 7.

GEFIs for measuring kinase activity.

Name	Analyte	Permutant	Sensor Unit	λex, nm	λem, nm	RA	Reference
blueAKAR	PKA activity	cpBFP	PKA substrate/ FHA1 from AKAR	385	450	−21% (in vivo)	[28]
blueCKAR	PKC activity	cpBFP	PKC substrate/FHA1 from AKAR	385	450	−23% (in vivo)	[28]
cyan-sinphos	Insulin induced protein phosphorylation	cpECFP	PH-PTB/SH2n	NM	482	−10% (in vivo)	[212]
green-sinphos	Insulin induced protein phosphorylation	cpEGFP	PH-PTB/SH2n	NM	515	15% (in vivo)	[212]
ExRai-AKAR	PKA activity	cpGFP	PKA substrate/FHA1 from AKAR	400/509	515	185% (in vivo)	[28]
ExRai-CKAR	PKC activity	cpGFP	PKC substrate/FHA1 from AKAR	400/509	515	155% (in vivo)	[28]
ExRai-AktAR	PKB activity	cpGFP	PKB substrate/FHA1 from AKAR	400/509	515	93% (in vivo)	[28]
yellow-sinphos	Insulin induced protein phosphorylation	cpCitrine	PH-PTB/SH2n	NM	NM	15% (in vivo)	[212]
sapphireAKAR	PKA activity	cp-T-sapphire	PKA substrate/FHA1 from AKAR	400	513	−25% (in vivo)	[28]
sapphireCKAR	PKC activity	cp-T-sapphire	PKC substrate/FHA1 from AKAR	400	513	−33% (in vivo)	[28]

The raw colors refer to the colors of fluorescent proteins used as reporter domains. NM—not measured. RA—response amplitude.