Table 8.
GEFIs for neurotransmitters measuring.
| Name | Analyte | Permutant | Sensor Unit | λex, nm | λem,nm | Kd | RA | Reference |
|---|---|---|---|---|---|---|---|---|
| SF-Azurite-iGluSnFR | Glutamate | cpAzurite | E. coli GltI | 370 | 450 | 62 ± 11 μM (in vitro) | NM | [102] |
| iGluSnFR | Glutamate | cpGFP | E. coli GltI | NM | 515 | 107 μM (in vitro) | 450% | [84] |
| SF-iGluSnFR | Glutamate | cpSF-GFP | E. coli GltI | 400 | 510 | 40 μM (in vitro) | NM | [102] |
| SF-iGluSnFR.A184S | Glutamate | cpSF-GFP | E. coli GltI | NM | NM | 7 μM (in vitro) | NM | [102] |
| SF-iGluSnFR.S72A | Glutamate | cpSF-GFP | E. coli GltI | NM | NM | 200 μM(in vitro) | NM | [102] |
| iGABASnFR | GABA | cpSF-GFP | Pf622 | NM | NM | 9 μM (in vitro) | 250% | [85] |
| iGABASnFRF.102Y.Y137L | GABA | cpSF-GFP | Pf622 | NM | NM | 70 μM (in vitro) | 350% | [85] |
| iGABASnFRF.102G | GABA | cpSF-GFP | Pf622 | NM | NM | 50 μM (in vitro) | 450% | [85] |
| dLight1.1 | Dopamine | cpGFP | DRD1 | NM | 516 | 330 nM | 230% | [82] |
| dLight1.2 | Dopamine | cpGFP | DRD1 | NM | 516 | 770 nM | 340% | [82] |
| dLight1.3a | Dopamine | cpGFP | DRD1 | NM | NM | 2.3 μM | 660% | [82] |
| dLight1.3b | Dopamine | cpGFP | DRD1 | NM | NM | 1.68 μM | 930% | [82] |
| dLight1.4 | Dopamine | cpGFP | DRD4 | NM | NM | 4.1 nM | 170% | [82] |
| dLight1.5 | Dopamine | cpGFP | DRD2 | NM | NM | 110 nM | 180% | [82] |
| B1AR-cpGFP | β-1 adrenergic receptors agonists | cpGFP | B1AR | NM | NM | NM | 70% | [82] |
| B2AR-cpGFP | β-2 adrenergic receptors agonists | cpGFP | B2AR | NM | NM | NM | 150% | [82] |
| KOR-cpGFP | κ-type opioid receptors agonists | cpGFP | KOR | NM | NM | NM | 80% | [82] |
| MOR-cpGFP | μ-type opioid receptors agonists | cpGFP | MOR | NM | NM | NM | 60% | [82] |
| A2AR-cpGFP | α-2 adrenergic receptors agonists | cpGFP | A2AR | NM | NM | NM | 40% | [82] |
| 5HT2A-cpGFP | Serotonin receptor-2A agonists | cpGFP | 5HT2A | NM | NM | NM | 40% | [82] |
| MT2-cpGFP | Melatonin type-2 receptor agonists | cpGFP | MT2 | NM | NM | NM | 40% | [82] |
| GACh1.0 | Acetylcholine | cpGFP | M3 | NM | NM | NM | 30% | [83] |
| GACh1.5 | Acetylcholine | cpGFP | M3 | NM | NM | NM | 70% | [83] |
| GACh2.0 | Acetylcholine | cpGFP | M3 | NM | NM | EC50 = 0.7 μM | 90% | [83] |
| GRABDA1m | Dopamine | cpEGFP | DRD2 | NM | NM | EC50 = 130 nM | 90% | [88] |
| GRABDA1h | Dopamine | cpEGFP | DRD2 | NM | NM | EC50 = 10 nM | 90% | [88] |
| GRABNE1m | Norepinephrine | cpEGFP | A2AR | NM | NM | 930 nM | 230% | [89] |
| GRABNE1h | Norepinephrine | cpEGFP | A2AR | NM | NM | 83 nM | 130% | [89] |
| SF-Venus-iGluSnFR | Glutamate | cpVenus | E. coli GltI | 510 | 520 | 27 ± 2 μM (in vitro) | [102] | |
| R-iGluSnFR1 | Glutamate | cpmApple | E. coli GltI | 562 | 588 | 11 μM (in vitro) | 4.9-fold | [58] |
The raw colors refer to the colors of fluorescent proteins used as reporter domains. Kd—dissociation constant. NM—not measured. RA—response amplitude.