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. 2019 Aug 24;20(17):4141. doi: 10.3390/ijms20174141

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Amino acid sequence from Populus trichocarpa with other species. (A) Sequence alignment of PtrARF2.1 in P. trichocarpa with AtARF2 in Arabidopsis thaliana. Peptide sequences were aligned by DNAMAN software. Identical and similar amino acid residues are indicated by dark and blue background, respectively. Numbers on the right refer to amino acid sequence positions. These conserved DBD/B3, ARF and Aux/IAA domains in ARF2 protein are underlined. (B) Phylogenetic analysis of the poplar and selected ARF2 proteins was performed by the neighbour-joining method using MEGA version 5. Bootstrap support is indicated at each node. Bar, 0.05 substitutions per site. Additional sequences include A. thaliana (AtARF2), Solanum lycopersicum (SlARF2A and SlARF2B), Vitis vinifera (VvARF2), Eucalyptus grandis (EgrARF2A and EgrARF2B). The accession numbers of ARF factor sequences are given in the Materials and Methods section.