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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biol Psychiatry. 2018 Jul 5;85(3):189–201. doi: 10.1016/j.biopsych.2018.06.019

Figure 2.

Figure 2.

Chronic restraint stress (CRS) remodels dendritic spines in basolateral amygdala (BLA) projection neurons (PNs) not targeting dorsomedial prefrontal cortex (dmPFC) (BLA↛dmPFC) but not BLA PNs targeting dmPFC (BLA→dmPFC). (A) Representative images of dendritic spines in BLA→dmPFC and BLA↛dmPFC PNs from unstressed control mice and CRS-exposed mice (scale bar = 2 μm). (B) Average spine density in BLA→dmPFC and BLA↛dmPFC PNs. PN group 3 CRS two-way analysis of variance: interaction (F1,31 = 1.21, p = .281), main effect of PN group (F1,31 = 0.54, p = .466), main effect of CRS (F1,31 = 2.53, p = .122). For BLA→dmPFC PNs, n = 9 neurons/8 control mice, n = 9 neurons/8 CRS-exposed mice. For BLA↛dmPFC PNs, n = 10 neurons/9 control mice, n = 7 neurons/7 CRS-exposed mice. (C) Cumulative probability of spine head diameter in BLA→dmPFC PNs. Control mice, n = 4108 spines/9 neurons; CRS-exposed mice, n = 4240 spines/9 neurons. Kolmogorov-Smirnov test: p = .956. Inset shows the average spine head diameter. t test: p = .123. (D) Cumulative probability of spine head diameter in BLA↛dmPFC PNs. Control mice, n = 5348 spines/10 neurons; CRS-exposed mice, n = 3459 spines/7 neurons. Kolmogorov-Smirnov test: p < .001. Inset shows the average spine head diameter. t test: **p < .01. (E, F) Average density in mushroom, thin, and stubby spines in BLA→dmPFC (E) and BLA↛dmPFC (F) PNs. t test: **p < .01. Data are means ± SEM.