FIG 3.
Barcodes from follow-up ESBL-KP isolates (black lines) compared to the corresponding first (gray lines) ESBL-KP ST101 isolate collected. The vertical gray (initial) and black (later) lines in the shaded region show where the Cas9 assay predicts the location of the blaCTX-M-15 gene. The dashed region indicates the suggested inversion in sample P6K25. For the larger plasmid regions, 5 to 65 kbp in size are missing in four of six isolates, and in one isolate, the plasmid is missing completely. P13K9 contained three plasmids, two of which are shown in the figure and none of the three are the same as the plasmids in the initial P13K0 isolate. Furthermore, we did not identify a blaCTX-M-15 gene in any of these three plasmids.