TABLE 1.

Quinone content of WT and Δyhb strains cultured in SMGN either aerobically or anaerobically^a

Culture condition	Strain	Quinone content
		UQ8 (pmol/mg cells)	DMK8 (247-nm peak area/mg cells)	MK8 (247-nm peak area/mg cells)
Air	WT	208 ± 9.4	0.94 ± 0.26	0.15 ± 0.09
Air	ΔyhbT	193.9 ± 12.4	1.34 ± 0.05	0.13 ± 0.03
Air	ΔyhbU	233.8 ± 16.2	0.9 ± 0.26	0.16 ± 0.09
Air	ΔyhbV	227.9 ± 25.1	0.93 ± 0.15	0.13 ± 0.04
Anaerobic	WT	114.2 ± 19.1	1.28 ± 0.4	0.41 ± 0.15
Anaerobic	ΔyhbT	3.3 ± 0.4	2.88 ± 0.47	0.17 ± 0.01
Anaerobic	ΔyhbU	6.1 ± 3.3	2.55 ± 0.55	0.19 ± 0.01
Anaerobic	ΔyhbV	7.3 ± 4.7	2.61 ± 0.49	0.21 ± 0.01
Anaerobic	ΔyhbT+pK	2 ± 0.4	2.46 ± 0.33	0.16 ± 0.04
Anaerobic	ΔyhbT+pK-yhbT	64.6 ± 16.1	1.77 ± 0.29	0.3 ± 0.04
Anaerobic	ΔyhbU+pBAD	1 ± 0.6	2.29 ± 0.58	0.18 ± 0.04
Anaerobic	ΔyhbU+pBAD-yhbU	82 ± 15.9	1.2 ± 0.18	0.27 ± 0.04
Anaerobic	ΔyhbV+pBAD	2.2 ± 3.5	2.14 ± 0.15	0.23 ± 0.01
Anaerobic	ΔyhbV+pBAD-yhbV	120.4 ± 18.5	1.87 ± 0.19	0.42 ± 0.06

^an = 3 to 5. For strains containing pK or pBAD vectors, SMGN was supplemented with 0.02% arabinose.