FIG 3.
Comparison of cell envelope components in WT and ΔibaG V. cholerae. (A and B) Abundance (A) and composition (B) of peptidoglycan (PG) isolated from exponential-phase WT and ΔibaG V. cholerae. PG from each strain was analyzed in triplicate. Monomers, dimers, and trimers represent muropeptides, and Di (dimers), Tri (trimers), Tetra (tetramers), and Penta (pentamers) represent peptides. *, P value of <0.01 by t test. (C) Quantification of different lengths and saturated forms of phosphatidylethanolamines (PEs), phosphatidylglycerols (PGlys), cardiolipins (CLs), lyso-PEs (L-PE), and lyso-PGs (L-PGlys) in whole-cell pellets from exponential-phase WT and ΔibaG V. cholerae. *, P value of <0.01 by t test. (D) Silver stained SDS-PAGE of LPS isolated from exponential phase from WT and ΔibaG strains.
